Construct design, biophysical, and biochemical characterization of the fusion core from mouse hepatitis virus (a coronavirus) spike protein.
Identifieur interne : 005411 ( Main/Exploration ); précédent : 005410; suivant : 005412Construct design, biophysical, and biochemical characterization of the fusion core from mouse hepatitis virus (a coronavirus) spike protein.
Auteurs : Yanhui Xu [République populaire de Chine] ; David K. Cole ; Zhiyong Lou ; Yiwei Liu ; Lan Qin ; Xu Li ; Zhihong Bai ; Fang Yuan ; Zihe Rao ; George F. GaoSource :
- Protein expression and purification [ 1046-5928 ] ; 2004.
Descripteurs français
- KwdFr :
- Chromatographie sur gel, Dichroïsme circulaire, Données de séquences moléculaires, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (), Masse moléculaire, Protéines de fusion virale (), Protéines de fusion virale (isolement et purification), Protéines de fusion virale (métabolisme), Protéines de l'enveloppe virale (), Structure secondaire des protéines, Séquence d'acides aminés, Virus de l'hépatite murine (), Virus de l'hépatite murine (pathogénicité).
- MESH :
- isolement et purification : Protéines de fusion virale.
- métabolisme : Protéines de fusion virale.
- pathogénicité : Virus de l'hépatite murine.
- Chromatographie sur gel, Dichroïsme circulaire, Données de séquences moléculaires, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires, Masse moléculaire, Protéines de fusion virale, Protéines de l'enveloppe virale, Structure secondaire des protéines, Séquence d'acides aminés, Virus de l'hépatite murine.
English descriptors
- KwdEn :
- Amino Acid Sequence, Chromatography, Gel, Circular Dichroism, Membrane Glycoproteins (chemistry), Molecular Sequence Data, Molecular Weight, Murine hepatitis virus (chemistry), Murine hepatitis virus (pathogenicity), Protein Structure, Secondary, Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (chemistry), Viral Fusion Proteins (chemistry), Viral Fusion Proteins (isolation & purification), Viral Fusion Proteins (metabolism).
- MESH :
- chemical , chemistry : Membrane Glycoproteins, Viral Envelope Proteins, Viral Fusion Proteins.
- chemistry : Murine hepatitis virus.
- chemical , isolation & purification : Viral Fusion Proteins.
- chemical , metabolism : Viral Fusion Proteins.
- pathogenicity : Murine hepatitis virus.
- Amino Acid Sequence, Chromatography, Gel, Circular Dichroism, Molecular Sequence Data, Molecular Weight, Protein Structure, Secondary, Spike Glycoprotein, Coronavirus.
Abstract
Membrane fusion between virus and host cells is the key step for enveloped virus entry and is mediated by the viral envelope fusion protein. In murine coronavirus, mouse hepatitis virus (MHV), the spike (S) protein mediates this process. Recently, the formation of anti-parallel 6-helix bundle of the MHV S protein heptad repeat (HR) regions (HR1 and HR2) has been confirmed, implying coronavirus has a class I fusion protein. This bundle is also called fusion core. To facilitate the solution of the crystal structure of this fusion core, we deployed an Escherichia coli in vitro expression system to express the HR1 and HR2 regions linked together by a flexible linker as a single chain (named 2-helix). This 2-helix polypeptide subsequently assembled into a typical 6-helix bundle. This bundle has been analyzed by a series of biophysical and biochemical techniques and confirmed that the design technique can be used for coronavirus as we successfully used for members of paramyxoviruses.
DOI: 10.1016/j.pep.2004.08.005
PubMed: 15477089
Affiliations:
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Le document en format XML
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Cole</name></author><author><name sortKey="Lou, Zhiyong" sort="Lou, Zhiyong" uniqKey="Lou Z" first="Zhiyong" last="Lou">Zhiyong Lou</name></author><author><name sortKey="Liu, Yiwei" sort="Liu, Yiwei" uniqKey="Liu Y" first="Yiwei" last="Liu">Yiwei Liu</name></author><author><name sortKey="Qin, Lan" sort="Qin, Lan" uniqKey="Qin L" first="Lan" last="Qin">Lan Qin</name></author><author><name sortKey="Li, Xu" sort="Li, Xu" uniqKey="Li X" first="Xu" last="Li">Xu Li</name></author><author><name sortKey="Bai, Zhihong" sort="Bai, Zhihong" uniqKey="Bai Z" first="Zhihong" last="Bai">Zhihong Bai</name></author><author><name sortKey="Yuan, Fang" sort="Yuan, Fang" uniqKey="Yuan F" first="Fang" last="Yuan">Fang Yuan</name></author><author><name sortKey="Rao, Zihe" sort="Rao, Zihe" uniqKey="Rao Z" first="Zihe" last="Rao">Zihe Rao</name></author><author><name sortKey="Gao, George F" sort="Gao, George F" uniqKey="Gao G" first="George F" last="Gao">George F. Gao</name></author></analytic><series><title level="j">Protein expression and purification</title><idno type="ISSN">1046-5928</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Chromatography, Gel</term><term>Circular Dichroism</term><term>Membrane Glycoproteins (chemistry)</term><term>Molecular Sequence Data</term><term>Molecular Weight</term><term>Murine hepatitis virus (chemistry)</term><term>Murine hepatitis virus (pathogenicity)</term><term>Protein Structure, Secondary</term><term>Spike Glycoprotein, Coronavirus</term><term>Viral Envelope Proteins (chemistry)</term><term>Viral Fusion Proteins (chemistry)</term><term>Viral Fusion Proteins (isolation & purification)</term><term>Viral Fusion Proteins (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Chromatographie sur gel</term><term>Dichroïsme circulaire</term><term>Données de séquences moléculaires</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires ()</term><term>Masse moléculaire</term><term>Protéines de fusion virale ()</term><term>Protéines de fusion virale (isolement et purification)</term><term>Protéines de fusion virale (métabolisme)</term><term>Protéines de l'enveloppe virale ()</term><term>Structure secondaire des protéines</term><term>Séquence d'acides aminés</term><term>Virus de l'hépatite murine ()</term><term>Virus de l'hépatite murine (pathogénicité)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term><term>Viral Fusion Proteins</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Murine hepatitis virus</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Viral Fusion Proteins</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Protéines de fusion virale</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Viral Fusion Proteins</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Protéines de fusion virale</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Murine hepatitis virus</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Virus de l'hépatite murine</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Chromatography, Gel</term><term>Circular Dichroism</term><term>Molecular Sequence Data</term><term>Molecular Weight</term><term>Protein Structure, Secondary</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Chromatographie sur gel</term><term>Dichroïsme circulaire</term><term>Données de séquences moléculaires</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires</term><term>Masse moléculaire</term><term>Protéines de fusion virale</term><term>Protéines de l'enveloppe virale</term><term>Structure secondaire des protéines</term><term>Séquence d'acides aminés</term><term>Virus de l'hépatite murine</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Membrane fusion between virus and host cells is the key step for enveloped virus entry and is mediated by the viral envelope fusion protein. In murine coronavirus, mouse hepatitis virus (MHV), the spike (S) protein mediates this process. Recently, the formation of anti-parallel 6-helix bundle of the MHV S protein heptad repeat (HR) regions (HR1 and HR2) has been confirmed, implying coronavirus has a class I fusion protein. This bundle is also called fusion core. To facilitate the solution of the crystal structure of this fusion core, we deployed an Escherichia coli in vitro expression system to express the HR1 and HR2 regions linked together by a flexible linker as a single chain (named 2-helix). This 2-helix polypeptide subsequently assembled into a typical 6-helix bundle. This bundle has been analyzed by a series of biophysical and biochemical techniques and confirmed that the design technique can be used for coronavirus as we successfully used for members of paramyxoviruses.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><noCountry><name sortKey="Bai, Zhihong" sort="Bai, Zhihong" uniqKey="Bai Z" first="Zhihong" last="Bai">Zhihong Bai</name><name sortKey="Cole, David K" sort="Cole, David K" uniqKey="Cole D" first="David K" last="Cole">David K. Cole</name><name sortKey="Gao, George F" sort="Gao, George F" uniqKey="Gao G" first="George F" last="Gao">George F. Gao</name><name sortKey="Li, Xu" sort="Li, Xu" uniqKey="Li X" first="Xu" last="Li">Xu Li</name><name sortKey="Liu, Yiwei" sort="Liu, Yiwei" uniqKey="Liu Y" first="Yiwei" last="Liu">Yiwei Liu</name><name sortKey="Lou, Zhiyong" sort="Lou, Zhiyong" uniqKey="Lou Z" first="Zhiyong" last="Lou">Zhiyong Lou</name><name sortKey="Qin, Lan" sort="Qin, Lan" uniqKey="Qin L" first="Lan" last="Qin">Lan Qin</name><name sortKey="Rao, Zihe" sort="Rao, Zihe" uniqKey="Rao Z" first="Zihe" last="Rao">Zihe Rao</name><name sortKey="Yuan, Fang" sort="Yuan, Fang" uniqKey="Yuan F" first="Fang" last="Yuan">Fang Yuan</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Xu, Yanhui" sort="Xu, Yanhui" uniqKey="Xu Y" first="Yanhui" last="Xu">Yanhui Xu</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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